Help icon

Detection Image Detection method details

Name:

 QT-EVE-DBN9501-qF/DBN9501-qR

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event DBN9501 (Wang et al., 2023)

Comment:

 Target is the 3' integration border region (IBR) between the insert of maize event DBN9501 and the maize host genome (included 65 bp T-DNA and 31 bp maize genome DNA sequence).

Validation:

 ring trial validation

Standardisation:

 unknown

Related Methods:

 QT-TAX-zSSIIb-3F/zSSIIb-4R

Type:

 event-specific

Target GMO name:

 DBN9501

  • Oligonucleotides:

  • Forward Primer

  • Name:

    DBN9501-qF

  • Sequence:

    AACGTGACTCCCTTAATTCTCC

  • Size:

    22

  • Reverse Primer

  • Name:

    DBN9501-qR

  • Sequence:

    CCGGACTACTATACCATTTATAGTTACA

  • Size:

    28

  • Probe

  • Name:

    DBN9501-qP

  • Sequence:

    ACTGAAGGCGGGAAACGACAATCT

  • Size:

    24

  • Amplicon:

  • Sequence:

    aacgtgactcccttaattctccgctcatgatcagattgtcgtttcccgccttcagtttaaactatcagtgtaactataaatggtatagtagtccgg

  • Size:

    96

Documents

Citation Type Local copy
Wang J, Xiao B, Zhang R, Guan H, Yang Y, Ding Y, Yuan R, Zhang X, Li L, Liang J, Wen H (2023) Inter-laboratory validation by event-specific qPCR methods for the detection of genetically modified insect and herbicide-tolerant maize DBN9501. Journal of Consumer Protection and Food Safety 18, 451-460Link to the document url. peer-reviewed article Download PDF pdf