Detection method details
Name: |
QT-EVE-BN-012 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event LBFLFK (EU-RL GMFF GMOMETHODS database as of October 12, 2022) |
Comment: |
Target is the 3' integration border region (IBR) between the insert of oilseed rape event LBFLFK locus1 and the oilseed rape host genome. |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-BN-001 |
Type: |
event-specific |
Target GMO name: |
LBFLFK |
-
Oligonucleotides:
Forward Primer
Name:
leftFST_ev1_loc1-f4Sequence:
ACCATCATACTCATTGCTGATCCSize:
23
Reverse Primer
Name:
leftFST_ev1_loc1-r5Sequence:
TTCCTGTATACGCACACATAGTATASize:
25
Probe
Name:
leftFST_ev1_loc1-s2Sequence:
ATGTAGATTTCCCGGACATGAAGCCASize:
26
-
Amplicon:
Sequence:
accatcatac tcattgctga tccatgtaga tttcccggac atgaagccan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntatacta tgtgtgcgta tacaggaaSize:
128
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-012 | EU reference method protocol |