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Detection Image Detection method details

Name:

 QT-EVE-GM-004

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean event A2704-12 (EU-RL GMFF GMOMETHODS database as of October 02, 2014)

Comment:

 Target is a junction region comprising an inverted repeat of CS-bla-ECOLX in soybean event A2704-12: 3' bla sequence and an inverted 5' bla sequence

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GM-009

Type:

 event-specific

Target GMO name:

 A2704-12

  • Oligonucleotides:

  • Forward Primer

  • Name:

    KVM175

  • Sequence:

    GCAAAAAAGCGGTTAGCTCCT

  • Size:

    21

  • Reverse Primer

  • Name:

    SMO001

  • Sequence:

    ATTCAGGCTGCGCAACTGTT

  • Size:

    20

  • Probe

  • Name:

    TM031

  • Sequence:

    CGGTCCTCCGATCGCCCTTCC

  • Size:

    21

  • Amplicon:

  • Sequence:

    gcaaaaaagcggttagctcctncggtcctccgatcgcccttccnaacagttgcgcagcctgaat

  • Size:

    64

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-004Link to the document url. EU reference method protocol