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Detection Image Detection method details

Name:

 QL-ELE-00-009

Description:

 Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)

Comment:

 Target is the nopaline synthase terminator (T-nos) from Agrobacterium tumefaciens

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 element-specific

Target DNA element:
T-nos-RHIRD

  • Oligonucleotides:

  • Forward Primer

  • Name:

    HA-nos118-f

  • Sequence:

    GCATGACGTTATTTATGAGATGGG

  • Size:

    24

  • Reverse Primer

  • Name:

    HA-nos118-r

  • Sequence:

    GACACCGCGCGCGATAATTTATCC

  • Size:

    24

  • Amplicon:

  • Sequence:

    gcatgacgttatttatgagatgggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnggataaattatcgcgcgcggtgtc

  • Size:

    118

Documents

Citation Type Local copy