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Detection Image Detection method details

Name:

 QL-EVE-ZM-P3waxy/P2waxy

Description:

 Qualitative real-time PCR for the detection of maize event GE-CRISPR-Cas Waxy Corn (Gao et al., 2020).

Comment:

 Target is the 6 kb deletion junction in the maize waxy gene locus.

Based on NCBI Reference Sequence: NC_050104.1 (Zm-B73-REFERENCE-NAM-5.0, whole genome shotgun sequence) forward primer P3 includes a 3 bp insertion (indicated in P3 as small letters) not complementary to the reference sequence. This might be explained by differences in the PH184C inbred line (mother line for GE-CRISPR-Cas Waxy Corn) compared to the B73 reference genome.

Validation:

 unknown

Standardisation:

 none

Related Methods:

Type:

 event-specific

Target GMO name:

 GE-CRISPR-Cas Waxy Corn

  • Oligonucleotides:

  • Forward Primer

  • Name:

    P3waxy

  • Sequence:

    ATGGAGAtcaGGTTAAGACAGCAAT

  • Size:

    25

  • Reverse Primer

  • Name:

    P2waxy

  • Sequence:

    AGCAGGGATTATTTACTCCACCAC

  • Size:

    24

  • Probe

  • Name:

    Probe2waxy

  • Sequence:

    AATTTGATGCCGTCCTAT

  • Size:

    18

  • Amplicon:

  • Sequence:

  • Size:

    145

Documents

Citation Type Local copy
Gao, H., Gadlage, M.J., Lafitte, H.R., Lenderts, B., Yang, M., Schroder, M., Farrell, J., Snopek, K., Peterson, D., Feigenbutz, L., Jones, S., St Clair, G., Rahe, M., Sanyour-Doyel, N., Peng, C., Wang, L., Young, J.K., Beatty, M., Dahlke, B., Hazebroek, J., Greene, T.W., Cigan, A.M., Chilcoat, N.D., Meeley, R.B., (2020) Superior field performance of waxy corn engineered using CRISPR–Cas9. Nature 38, 579-581.Link to the document url. peer-reviewed article