Detection method details
Name: |
QL-EVE-SQ0718/SQ0719 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of wheat event MON71800 (verification report of the EU-RL GMFF, 4 Dec 2013). |
Comment: |
Target is the region spanning the insert-to-plant junction in wheat event MON71800. The probe is labelled with a MGB-NFQ (minor groove binding non-fluorescent quencher) as quencher at its 3’ end. |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
QT-TAX-wx012-5'/wx012-3' |
Type: |
event-specific |
Target GMO name: |
MON71800 |
-
Oligonucleotides:
Forward Primer
Name:
SQ0718Sequence:
TTCTTCTCTCTCTTTGAATCTCAATACAASize:
29
Reverse Primer
Name:
SQ0719Sequence:
CCCCCATTTGGACGTGAASize:
18
Probe
Name:
PB0101Sequence:
TCCCCCTCTCTAATTCSize:
16
-
Amplicon:
Sequence:
ttcttctctctctttgaatctcaatacaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttcacgtccaaatgggggSize:
95
Documents
Citation | Type | Local copy |
---|---|---|
Report on the Verification of the Performance of a Method for the Detection of Event MON71800 in Wheat Using Real-Time PCR (EURL-GMFF 4 Dec 2013) | EU reference method protocol |