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Detection Image Detection method details

Name:

 QL-ELE-P-SSuAra-F/P-SSuAra-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of SSUAra promoter (ribulose 1.5-biphoshate carboxylase small subunit) of Arabidopsis thaliana (Debode et al., 2013)

Comment:

 Target is the SSUAra promoter (P-rbcS) from Arabidopsis thaliana

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
P-rbcS-ARATH

  • Oligonucleotides:

  • Forward Primer

  • Name:

    P-SSuAra-F

  • Sequence:

    GGCCTAAGGAGAGGTGTTGAGA

  • Size:

    22

  • Reverse Primer

  • Name:

    P-SSuAra-R

  • Sequence:

    CTCATAGATAACGATAAGATTCATGGAATT

  • Size:

    30

  • Probe

  • Name:

    P-SSuAra-P

  • Sequence:

    CCTTATCGGCTTGAACCGCTGGAATAA

  • Size:

    27

  • Amplicon:

  • Sequence:

    GGCCTAAGGAGAGGTGTTGAGACCCTTATCGGCTTGAACCGCTGGAATAATGCCACGTGGAAGATAATTCCATGAATCTTATCGTTATCTATGAG

  • Size:

    95

Documents

Citation Type Local copy