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Detection Image Detection method details

Name:

 QL-EVE-BN-002

Description:

 Qualitative conventional PCR method for the detection of oilseed rape event GT73 (ISO 21569).

Comment:

 target is the 3' integration border region (IBR) between the E9 gene terminator (T-E9) of oilseed rape event GT73 and the oilseed rape host genome

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

 QL-TAX-BN-004

Type:

 event-specific

Target GMO name:

 GT73

  • Oligonucleotides:

  • Forward Primer

  • Name:

    RT73-1

  • Sequence:

    AATAACGCTGCGGACATCTA

  • Size:

    20

  • Reverse Primer

  • Name:

    RT73-2

  • Sequence:

    CAGCAAGATTCTCTGTCAACAA

  • Size:

    22

  • Amplicon:

  • Sequence:

    aataacgctgcggacatctannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttgttgacagagaatcttgctg

  • Size:

    204

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-EVE-BN-002Link to the document url. EU reference method protocol
Pan L., Zhang S., Yang L., Broll H., Tian F., Zhang D.; Interlaboratory trial validation of an event-specific qualitative polymerase chain reaction-based detection method for genetically modified RT73 rapeseed J AOAC Int 90:1639-1646 (2007)Link to the document url. peer-reviewed article
Pan L., Zhang S., Yang L., Broll H., Tian F., Zhang D.; Interlaboratory trial validation of an event-specific qualitative polymerase chain reaction-based detection method for genetically modified RT73 rapeseed" J AOAC Int 90:1639-1646 (2007)Link to the document url.