Detection method details
Name: |
QL-ELE-AINT 2-5'/AINT 2-3' |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 intron (I-ractI) (Mano et al. 2009). |
Comment: |
Target is the actin 1 intron from rice (Oryza sativa). |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
I-actin
|
-
Oligonucleotides:
Forward Primer
Name:
AINT 2-5'Sequence:
TCGTCAGGCTTAGATGTGCTAGASize:
23
Reverse Primer
Name:
AINT 2-3'Sequence:
CTGCATTTGTCACAAATCATGAASize:
23
Probe
Name:
AINT-2-TaqSequence:
TTTGTGGGTAGAATTTGAATCCCTCAGCSize:
28
-
Amplicon:
Sequence:
tcgtcaggcttagatgtgctagatctttctttcttctttttgtgggtagaatttgaatccctcagcattgttcatcggtagtttttcttttcatgatttgtgacaaatgcagSize:
112