Detection method details
Name: |
QL-ELE-T-g7-F/T-g7-R |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the g7 terminator from Agrobacterium tumefaciens (Debode et al., 2013) |
Comment: |
Target is the Agrobacterium tumefaciens g7 terminator (T-g7)
T-g7 (aka T-transcript7 or T-tr7) also recognises ORF23 of Agrobacterium tumefaciens since this is encoded by the reverse strand of T-g7. |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
T-transcript 7, T-orf23-RHIRD
|
-
Oligonucleotides:
Forward Primer
Name:
T-g7-FSequence:
ATGCAAGTTTAAATTCAGAAATATTTCAASize:
29
Reverse Primer
Name:
T-g7-RSequence:
ATGTATTACACATAATATCGCACTCAGTCTSize:
30
Probe
Name:
T-g7-PSequence:
ACTGATTATATCAGCTGGTACATTGCCGTAGATGASize:
35
-
Amplicon:
Sequence:
-Size:
97
Documents
Citation | Type | Local copy |
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