Detection method details
Name: |
QL-ELE-P-Ubi-F/P-Ubi-R |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of ubiquitin promoter of Zea mays (Debode et al., 2013) |
Comment: |
Target is the ubiquitin promoter (P-ubi) from Zea mays |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
P-ubi1-MAIZE
|
-
Oligonucleotides:
Forward Primer
Name:
P-Ubi-FSequence:
GAGTAGATAATGCCAGCCTGTTAAACSize:
26
Reverse Primer
Name:
P-Ubi-RSequence:
ACGCGACGCTGCTGGTTSize:
17
Probe
Name:
P-Ubi-PSequence:
CGTCGACGAGTCTAACGGACACCAACSize:
26
-
Amplicon:
Sequence:
GAGTAGATAATGCCAGCCTGTTAAACGCCGTCGACGAGTCTAACGGACACCAACCAGCGAACCAGCAGCGTCGCGTSize:
76