Detection method details
Name: |
QL-ELE-Cry1Ac-F(/R)-n4/Cry1AcR-n2 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the cry1Ac gene (Scholtens et al., 2013). |
Comment: |
Target is the cry1Ac gene from bacterium Bacillus thuringiensis. |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-cry1Ac-BACTU
|
-
Oligonucleotides:
Forward Primer
Name:
Cry1Ac-F(/R)-n4Sequence:
TTCAGGACCAGGATTCACSize:
18
Reverse Primer
Name:
Cry1AcR-n2Sequence:
GTGAATAGGGGTCACAGAAGCATASize:
24
Probe
Name:
Cry1AcP-n3Sequence:
TCTGGTAGATGTGGATGGGAAGTSize:
23
-
Amplicon:
Sequence:
ttcaggaccaggattcactggtggagacctcgttagactcaacagcagtggaaacaacattcagaatagagggtatattgaagttccaattcacttcccatccacatctaccagatatagagttcgtgtgaggtatgcttctgtgacccctattcacSize:
157