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Detection method details
Name: |
QL-ELE-T-OCS-F/T-OCS-R |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of octopine synthase terminator of Agrobacterium tumefaciens (Debode et al., 2013) |
Comment: |
Target is the octopine synthase terminator (T-OCS) from Agrobacterium tumefaciens.
There are two nucleotides missing in the reverse primer. The amplicon given is the actual sequence generated from Agrobacterium tumefaciens (GenBank: CP011249.1) T-OCS-R: CGCTCGGTGTCGTAGATACT (Debode et al., 2013) T-OCS-R: CGCTCGGTGTGTCGTAGATACT (corrected) |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
T-ocs-RHIRD
|
-
Oligonucleotides:
Forward Primer
Name:
T-OCS-FSequence:
CGGTCAAACCTAAAAGACTGATTACASize:
26
Reverse Primer
Name:
T-OCS-RSequence:
CGCTCGGTGTCGTAGATACTSize:
20
Probe
Name:
T-OCS-PSequence:
TCTTATTCAAATTTCAAAAGTGCCCCAGGGSize:
30
-
Amplicon:
Sequence:
CGGTCAAACCTAAAAGACTGATTACATAAATCTTATTCAAATTTCAAAAGTGCCCCAGGGGCTAGTATCTACGACACACCGAGCGSize:
85