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Detection Image Detection method details

Name:

 QL-ELE-cry1A.105 - F1/cry1A.105 - R1

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the cry1A.105 gene (Dinon et al. 2011).

Comment:

 Target is the synthetic cry1A.105 gene.

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
CS-cry1A_105-SYNTH

  • Oligonucleotides:

  • Forward Primer

  • Name:

    cry1A.105 - F1

  • Sequence:

    TCAGAGGTCCAGGGTTTACAGG

  • Size:

    22

  • Reverse Primer

  • Name:

    cry1A.105 - R1

  • Sequence:

    GTAGTAGAGGCATAGCGGATTCTTG

  • Size:

    25

  • Probe

  • Name:

    cry1A.105 - P

  • Sequence:

    AGACATTCTTCGTCGCACAAGTGGAGGACC

  • Size:

    30

  • Amplicon:

  • Sequence:

    tcagaggtccagggtttacaggaggagacattcttcgtcgcacaagtggaggaccctttgcttacactattgttaacatcaatggccaattgccccaaaggtatcgtgcaagaatccgctatgcctctactac

  • Size:

    133

Documents

Citation Type Local copy
Dinon, A. Z., T. W. Prins, et al. (2011). Development and validation of real-time PCR screening methods for detection of cry1A.105 and cry2Ab2 genes in genetically modified organisms." Analytical and Bioanalytical Chemistry 400(5): 1433-1442.Link to the document url. peer-reviewed article