Detection method details
Name: |
QT-EVE-GH-011 |
Description: |
Quantitative PCR method for detection of cotton event DAS81910 (EURL GMFF GMOMETHODS database as of January 10, 2019) |
Comment: |
Target is the 5' integration border region (IBR) between the insert of cotton event DAS81910 and the cotton host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-GH-016, QT-TAX-GH-021 |
Type: |
event-specific |
Target GMO name: |
DAS81910 |
-
Oligonucleotides:
Forward Primer
Name:
1706-f2Sequence:
AAGCTTAGGTGATTTCGATGATGSize:
23
Reverse Primer
Name:
1706-r3Sequence:
GACCTCAATTGCGAGCTTTCSize:
20
Probe
Name:
1706-p3Sequence:
CACACCAAAAGTTAGGCCCGSize:
20
-
Amplicon:
Sequence:
aagcttaggt gatttcgatg atgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncacac caaaagttag gcccgnnnnn nnnnnnnnnn gaaagctcgc aattgaggtcSize:
120
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-GH-011 | EU reference method protocol |