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Detection Image Detection method details

Name:

 QT-EVE-BN-007

Description:

 Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf2 (verified by the EU-RL GMFF in the context of Commission Decision 2007/306/EC)

Comment:

 Target is the 5'integration border region (IBR) between the insert of oilseed rape event RF2 and the oilseed rape host genome

Validation:

 in-house validation

Standardisation:

 unknown

Related Methods:

 QT-TAX-BN-012

Type:

 event-specific

Target GMO name:

 Rf2

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MDB207

  • Sequence:

    GGGTGAGACAATATATCGACG

  • Size:

    21

  • Reverse Primer

  • Name:

    KVM171

  • Sequence:

    GGGCATCGCACCGGTGAG

  • Size:

    18

  • Probe

  • Name:

    TM024

  • Sequence:

    CACCGGCCAAATTCGCTCTTAGCCGT

  • Size:

    26

  • Amplicon:

  • Sequence:

    gggtgagacaatatatcgacgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncaccggccaaattcgctcttagccgtnnnnnnnnnctcaccggtgcgatgccc

  • Size:

    104

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-007Link to the document url. EU reference method protocol