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Detection method details
Name: |
QL-CON-00-013 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the maize ubiquitin promoter and the modified cry1Ab/1Ac genes (Grohmann et al.,2015). |
Comment: |
Target is the junction region between the ubiquitin promoter (P-ubi) sequence from maize (Zea mays) and the cry1Ab/Ac genes from Bacillus thuringiensis. |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
construct-specific |
Target GMO names: |
3006-210-23, 4114, DAS1507, DAS59122, DAS59132, KMD1, Kefeng6
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Oligonucleotides:
Forward Primer
Name:
pubi-F2Sequence:
ATTTGCTTGGTACTGTTTCTTTTGTCSize:
26
Reverse Primer
Name:
cry-rr-RSequence:
TTGTTGTCCATGGATCCTCTAGAGTSize:
25
Probe
Name:
pubi-T2Sequence:
ACCCTGTTGTTTGGTGTTACTTCTGCASize:
27
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Amplicon:
Sequence:
atttgcttggtactgtttcttttgtcgatgctcaccctgttgtttggtgttacttctgcaggtcgactctagaggatccatggacaacaaSize:
90