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Detection Image Detection method details

Name:

 QT-ELE-00-004

Description:

 Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)

Comment:

 the target is the 35S promoter (P-35S) from Cauliflower mosaic virus

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 element-specific

Target DNA element:
E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH

  • Oligonucleotides:

  • Forward Primer

  • Name:

    35S-F

  • Sequence:

    GCCTCTGCCGACAGTGGT

  • Size:

    18

  • Reverse Primer

  • Name:

    35S-R

  • Sequence:

    AAGACGTGGTTGGAACGTCTTC

  • Size:

    22

  • Probe

  • Name:

    35S-TMP

  • Sequence:

    CAAAGATGGACCCCCACCCACG

  • Size:

    22

  • Amplicon:

  • Sequence:

    gcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtctt

  • Size:

    82

Documents

Citation Type Local copy
ISO21570:2005(E) Annex B (informative)Link to the document url. EU reference method protocol
EU-RL GMFF GMOMETHODS database; QT-ELE-00-004Link to the document url. EU reference method protocol