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Detection Image Detection method details

Name:

 QT-EVE-GH-002

Description:

 Quantitative real-time PCR (TaqMan) method for detection of cotton event LLCotton25 (EU-RL GMFF GMOMETHODS database as of January 13, 2014)

Comment:

 Target is the 5' integration border region (IBR) between the insert of cotton event LLCotton25 and the cotton host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GH-018

Type:

 event-specific

Target GMO name:

 LLCotton25

  • Oligonucleotides:

  • Forward Primer

  • Name:

    KVM156

  • Sequence:

    CAAGGAACTATTCAACTGAG

  • Size:

    20

  • Reverse Primer

  • Name:

    KVM155

  • Sequence:

    CAGATTTTTGTGGGATTGGAATTC

  • Size:

    24

  • Probe

  • Name:

    TM018

  • Sequence:

    CTTAACAGTACTCGGCCGTCGACCGC

  • Size:

    26

  • Amplicon:

  • Sequence:

    caaggaactattcaactgagcttaacagtactcggccgtcgaccgcnnnnnnnnngaattccaatcccacaaaaatctg

  • Size:

    79

Documents

Citation Type Local copy
Mazzara M., Grazioli E., Savini C., Van Den Eede G.; Event-Specific Method for the Quantification of Cotton Line LLCotton25" Using Real-time PCR - Validation Report and Protocol - Cotton Seeds Sampling and DNA Extraction" Online Publication (2007)Link to the document url. EU reference method protocol
EU-RL GMFF GMOMETHODS database; QT-EVE-GH-002Link to the document url. EU reference method protocol