Detection method details
Name: |
QL-ELE-PFMV 1-5'/PFMV 1-3' |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of P-FMV (Mano et al., 2009) |
Comment: |
Target is the 34S promoter sequence (P-FMV) from Figwort mosaic virus |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
P-34S FMV
|
-
Oligonucleotides:
Forward Primer
Name:
PFMV 1-5'Sequence:
ATCAACAAGGTACGAGCCATATCSize:
23
Reverse Primer
Name:
PFMV 1-3'Sequence:
TGAGGCTTTGGACTGAGAATTCSize:
22
Probe
Name:
PFMV-1-TaqSequence:
CCAAGAAGGAACTCCCATCCTCAAAGGTTTSize:
30
-
Amplicon:
Sequence:
ATCAACAAGGTACGAGCCATATCACTTTATTCAAATTGGTATCGCCAAAACCAAGAAGGAACTCCCATCCTCAAAGGTTTGTAAGGAAGAATTCTCAGTCCAAAGCCTCASize:
110