Detection method details
Name: |
QL-TAX-SS-AM5F/AM5R |
Description: |
Quanlitative real-time PCR (TaqMan) method for detection of Salmo salar (Atlantic salmon) (Soga et al. 2020) |
Comment: |
Target is the endogenous Growth hormone gene 1 (GH1) of Salmo salar.
The amplicon size according to Soga et al. ((2020) Food Chemistry 305:125426) is not correct. It should be 177 instead of 176 bp. |
Validation: |
in-house validation |
Standardisation: |
unknown |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Salmo salar |
-
Oligonucleotides:
Forward Primer
Name:
AM5FSequence:
AAGGTGCAAAACCATGTTGCCTTCTSize:
25
Reverse Primer
Name:
AM5RSequence:
ATGTGAGTGTTCTAGGTCACTAGACSize:
25
Probe
Name:
AM5PR-2Sequence:
TGCGTTTCTTGTGCTCTCTATTGCAAAGTASize:
30
-
Amplicon:
Sequence:
aaggtgcaaaaccatgttgccttctatttcatgtgccttcctatattttctacagtgcgtttcttgtgctctctattgcaaagtatctttgggtctttaacccatatattattactattattgttcattgatcaagactgttctcgagaaaggtctagtgacctagaacactcacatSize:
177
Documents
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