Detection method details
Name: |
QT-EVE-ZM-022 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize event MIR162 (EU-RL GMFF GMOMETHODS database as of January 10, 2014) |
Comment: |
Target is the 3' integration border region (IBR) between the insert of maize event MIR162 and the maize host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-ZM-014 |
Type: |
event-specific |
Target GMO name: |
MIR162 |
-
Oligonucleotides:
Forward Primer
Name:
MIR162-f1Sequence:
GCGCGGTGTCATCTATGTTACTAGSize:
24
Reverse Primer
Name:
MIR162-r1Sequence:
TGCCTTATCTGTTGCCTTCAGASize:
22
Probe
Name:
MIR162-p1Sequence:
TCTAGACAATTCAGTACATTAAAAACGTCCGCCASize:
34
-
Amplicon:
Sequence:
gcgcggtgtcatctatgttactagnnnnnnnnntctagacaattcagtacattaaaaacgtccgccannntctgaaggcaacagataaggcaSize:
92
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-022 | EU reference method protocol |