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Detection Image Detection method details

Name:

 QT-TAX-wx012-5'/wx012-3'

Description:

 Quantitative real-time PCR (TaqMan) method for detection of the common wheat waxy-D1 gene (Iida et al., 2005)

Comment:

 Target is the waxy-D1 gene coding for the granule-bound starch synthase from common wheat (Triticum aestivum)

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 taxon-specific

Target Species name:

 Triticum aestivum

  • Oligonucleotides:

  • Forward Primer

  • Name:

    wx012-5'

  • Sequence:

    GTCGCGGGAACAGAGGTGT

  • Size:

    19

  • Reverse Primer

  • Name:

    wx012-3'

  • Sequence:

    GGTGTTCCTCCATTGCGAAA

  • Size:

    20

  • Probe

  • Name:

    wx012-T

  • Sequence:

    CAAGGCGGCCGAAATAAGTTGCC

  • Size:

    23

  • Amplicon:

  • Sequence:

    GTCGCGGGAACAGAGGTGTTCAAGGCGGCCGAAATAAGTTGCCGCCTGCGGCGGAATCGCCACCCACCGTGAAGTTCACCGTTTCGCAATGGAGGAACACC

  • Size:

    102

Documents

Citation Type Local copy
Iida M, Yamashiro S, Yamakawa H, Hayakawa K, Kuribara H, Kodama T, Furui S, Akiyama H, Maitani T, Hino A. Development of taxon-specific sequences of common wheat for the detection of genetically modified wheat. J Agric Food Chem. 2005, 53(16):6294-300.Link to the document url.