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Detection Image Detection method details

Name:

 QT-EVE-GH-009

Description:

 Quantitative real-time PCR (TaqMan) method for detection of cotton event T304-40 (EU-RL GMFF GMOMETHODS database as of January 27, 2014)

Comment:

 Target is the 3' integration border region (IBR) between the insert of cotton event T304-40 and the cotton host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GH-019

Type:

 event-specific

Target GMO name:

 T304-40

  • Oligonucleotides:

  • Forward Primer

  • Name:

    SHA029

  • Sequence:

    AGCGCGCAAACTAGGATAAATT

  • Size:

    22

  • Reverse Primer

  • Name:

    SHA030

  • Sequence:

    CCTAGATCTTGGGATAACTTGAAAAGA

  • Size:

    27

  • Probe

  • Name:

    TM089

  • Sequence:

    TCGCGCGCGGTGTCATCTATCTC

  • Size:

    23

  • Amplicon:

  • Sequence:

    agcgcgcaaactaggataaattntcgcgcgcggtgtcatctatctcnnnnntcttttcaagttatcccaagatctagg

  • Size:

    78

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GH-009Link to the document url. EU reference method protocol