Detection method details
Name: |
QL-ELE-00-020 |
Description: |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-cry1Ab-BACTU (Barbau-Piednoir et al., 2014) |
Comment: |
Target is CS-cry1Ab-BACTU from Bacillus thuringiensis |
Validation: |
ring trial validation |
Standardisation: |
unknown |
Related Methods: |
QL-TAX-BN-003, QL-TAX-GM-001, QL-TAX-ZM-002 |
Type: |
element-specific |
Target DNA element: |
CS-cry1Ab-BACTU
|
-
Oligonucleotides:
Forward Primer
Name:
CryIAb_Bt_Cott_FwdSequence:
ACCGGTTACACTCCCATCGASize:
20
Reverse Primer
Name:
CryIAb_Bt_Cott_RevSequence:
CAGCACCTGGCACGAACTCSize:
19
-
Amplicon:
Sequence:
accggttacactcccatcgannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngagttcgtgccaggtgctgSize:
73
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QL-ELE-00-020 | EU reference method protocol | |
Barbau-Piednoir, E., Stragier, P., Roosens, N., Mazzara, M., Savini, C., Van den Eede, G., Van den Bulcke, M. (2014). Inter-laboratory testing of GMO detection by combinatory SYBR®Green PCR screening (CoSYPS). Food Analytical Methods. 7, 1719-1728. | peer-reviewed article |