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Detection Image Detection method details

Name:

 QT-TAX-BV-013

Description:

 Quantitative real-time PCR (TaqMan) method for detection of sugar beet glutamine synthetase (GS2) gene (EU-RL GMFF GMOMETHODS database as of March 05, 2014)

Comment:

 Target is the endogenous glutamine synthetase (GS2) gene from Beta vulgaris

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 taxon-specific

Target Species name:

 Beta vulgaris

  • Oligonucleotides:

  • Forward Primer

  • Name:

    GluA3-F

  • Sequence:

    GACCTCCATATTACTGAAAGGAAG

  • Size:

    24

  • Reverse Primer

  • Name:

    GluA3-R

  • Sequence:

    GAGTAATTGCTCCATCCTGTTCA

  • Size:

    23

  • Probe

  • Name:

    GluD1

  • Sequence:

    CTACGAAGTTTAAAGTATGTGCCGCTC

  • Size:

    27

  • Amplicon:

  • Sequence:

    gacctccatattactgaaaggaagnnnnnnnnnnnnnnnnnnnnnnnctacgaagtttaaagtatgtgccgctcnnnnnnnnnnnnnnnnnnnnntgaacaggatggagcaattactc

  • Size:

    118

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-TAX-BV-013Link to the document url. EU reference method protocol