Detection method details
Name: |
QL-ELE-00-022 |
Description: |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-bar-STRHY (Barbau-Piednoir et al., 2014) |
Comment: |
Target is CS-bar-STRHY from bacterium Streptomyces hygroscopicus |
Validation: |
ring trial validation |
Standardisation: |
unknown |
Related Methods: |
QL-TAX-BN-003, QL-TAX-GM-001, QL-TAX-ZM-002 |
Type: |
element-specific |
Target DNA element: |
CS-bar-STRHY
|
-
Oligonucleotides:
Forward Primer
Name:
Pat-Bar FwdSequence:
CGTCAACCACTACATCGAGACAASize:
23
Reverse Primer
Name:
Pat-Bar RevSequence:
GTCCACTCCTGCGGTTCCTSize:
19
-
Amplicon:
Sequence:
cgtcaaccactacatcgagacaannnnnnnnnnnnnnnnnnnnnnnnnnnaggaaccgcaggagtggacSize:
69
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QL-ELE-00-022 | EU reference method protocol | |
Barbau-Piednoir, E. et al. (2012). Four new SYBR_Green qPCR screening methods for the detection of Roundup Ready, LibertyLink, and CryIAb traits in genetically modified products. Eur Food Res Technol, 234, 13–23. | peer-reviewed article |