Detection method details
Name: |
QT-EVE-ZM-014 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize event GA21 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) |
Comment: |
Target is the 5' integration border region (IBR) between the insert of maize event GA21 and the maize host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-ZM-014 |
Type: |
event-specific |
Target GMO name: |
GA21 |
-
Oligonucleotides:
Forward Primer
Name:
esGA21-5' forward primerSequence:
CGTTATGCTATTTGCAACTTTAGAACASize:
27
Reverse Primer
Name:
esGA21-5' reverse primerSequence:
GCGATCCTCCTCGCGTTSize:
17
Probe
Name:
esGA21-5' probeSequence:
TTTCTCAACAGCAGGTGGGTCCGGGTSize:
26
-
Amplicon:
Sequence:
cgttatgctatttgcaactttagaacannnnnnnnnnnnnnnnnntttctcaacagcaggtgggtccgggtnnnnnnnnnnnnnaacgcgaggaggatcgcSize:
101
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-014 | EU reference method protocol |