Detection method details
Name: |
QL-EVE-ZM-P3waxy/P2waxy |
Description: |
Qualitative real-time PCR for the detection of maize event GE-CRISPR-Cas Waxy Corn (Gao et al., 2020). |
Comment: |
Target is the 6 kb deletion junction in the maize waxy gene locus.
Based on NCBI Reference Sequence: NC_050104.1 (Zm-B73-REFERENCE-NAM-5.0, whole genome shotgun sequence) forward primer P3 includes a 3 bp insertion (indicated in P3 as small letters) not complementary to the reference sequence. This might be explained by differences in the PH184C inbred line (mother line for GE-CRISPR-Cas Waxy Corn) compared to the B73 reference genome. |
Validation: |
unknown |
Standardisation: |
none |
Related Methods: |
Type: |
event-specific |
Target GMO name: |
GE-CRISPR-Cas Waxy Corn |
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Oligonucleotides:
Forward Primer
Name:
P3waxySequence:
ATGGAGAtcaGGTTAAGACAGCAATSize:
25
Reverse Primer
Name:
P2waxySequence:
AGCAGGGATTATTTACTCCACCACSize:
24
Probe
Name:
Probe2waxySequence:
AATTTGATGCCGTCCTATSize:
18
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Amplicon:
Sequence:
Size:
145