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Detection method details
Name: |
QL-ELE-BstarF-n2 /BstarR-n2 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the barstar gene (Xu et al., 2006) |
Comment: |
target is the barnase ribonuclease inhibitor (barstar) gene from bacterium Bacillus amyloliquefaciens |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-barstar
|
-
Oligonucleotides:
Forward Primer
Name:
BstarF-n2Sequence:
AACAAATCAGAAGTATCAGCGACCTSize:
25
Reverse Primer
Name:
BstarR-n2Sequence:
AACTGCCTCCATTCCAAAACGSize:
21
Probe
Name:
BStarP-n3Sequence:
ACCTGGACGCTTTATGGGATTSize:
21
-
Amplicon:
Sequence:
aacaaatcagaagtatcagcgacctccaccagacattgaaaaaggagcttgcccttccggaatactacggtgaaaacctggacgctttatgggattgtctgaccggatgggtggagtacccgctcgttttggaatggaggcagttSize:
145