Detection method details
Name: |
QT-TAX-BV-013 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of sugar beet glutamine synthetase (GS2) gene (EU-RL GMFF GMOMETHODS database as of March 05, 2014) |
Comment: |
Target is the endogenous glutamine synthetase (GS2) gene from Beta vulgaris |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Beta vulgaris |
-
Oligonucleotides:
Forward Primer
Name:
GluA3-FSequence:
GACCTCCATATTACTGAAAGGAAGSize:
24
Reverse Primer
Name:
GluA3-RSequence:
GAGTAATTGCTCCATCCTGTTCASize:
23
Probe
Name:
GluD1Sequence:
CTACGAAGTTTAAAGTATGTGCCGCTCSize:
27
-
Amplicon:
Sequence:
gacctccatattactgaaaggaagnnnnnnnnnnnnnnnnnnnnnnnctacgaagtttaaagtatgtgccgctcnnnnnnnnnnnnnnnnnnnnntgaacaggatggagcaattactcSize:
118
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-TAX-BV-013 | EU reference method protocol |