Help icon

Detection Image Detection method details

Name:

 QT-EVE-ZM-009

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event MON863 (EU-RL GMFF GMOMETHODS database as of March 13, 2014)

Comment:

 Target is the 5' integration border region (IBR) between the insert of maize event MON863 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-011

Type:

 event-specific

Target GMO name:

 MON863

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MON863 primer F

  • Sequence:

    TGTTACGGCCTAAATGCTGAACT

  • Size:

    23

  • Reverse Primer

  • Name:

    MON863 primer R

  • Sequence:

    GTAGGATCGGAAAGCTTGGTAC

  • Size:

    22

  • Probe

  • Name:

    MON863 Probe

  • Sequence:

    TGAACACCCATCCGAACAAGTAGGGTCA

  • Size:

    28

  • Amplicon:

  • Sequence:

    tgttacggcctaaatgctgaactnntgaccctacttgttcggatgggtgttcannnnnnnnngtaccaagctttccgatcctac

  • Size:

    84

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-009Link to the document url. EU reference method protocol