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Detection Image Detection method details

Name:

 QT-EVE-ST-001

Description:

 Quantitative real-time PCR (TaqMan) method for detection of potato event EH92-527-1 (EU-RL GMFF GMOMETHODS database as January 13, 2014)

Comment:

 Target is the 3' integration border region (IBR) between the insert of potato event EH92-527-1 and the potato host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 event-specific

Target GMO name:

 EH92-527-1

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Event527-bf1

  • Sequence:

    GTGTCAAAACACAATTTACAGCA

  • Size:

    23

  • Reverse Primer

  • Name:

    St527-R1

  • Sequence:

    TCCCTTAATTCTCCGCTCATGA

  • Size:

    22

  • Probe

  • Name:

    St527-S2

  • Sequence:

    AGATTGTCGTTTCCCGCCTTCAGTT

  • Size:

    25

  • Amplicon:

  • Sequence:

    gtgtcaaaacacaatttacagcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaactgaaggcgggaaacgacaatctnntcatgagcggagaattaaggga

  • Size:

    134

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ST-001Link to the document url. EU reference method protocol