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Detection Image Detection method details

Name:

 QT-EVE-GM-018

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean event GMB151 (EU-RL GMFF GMOMETHODS database as of August 10, 2020)

Comment:

 Target is the 5' integration border region (IBR) between the insert of soybean event GMB151 and the soybean host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GM-003

Type:

 event-specific

Target GMO name:

 GMB151

  • Oligonucleotides:

  • Forward Primer

  • Name:

    PRIM1040

  • Sequence:

    TCAAATCAACATGGGTGACTAGAAA

  • Size:

    25

  • Reverse Primer

  • Name:

    PRIM1041

  • Sequence:

    CATTGTGCTGAATAGGTTTATAGCTATGAT

  • Size:

    30

  • Probe

  • Name:

    TM1789

  • Sequence:

    CAGTACTGGGCCCTTGTGGCGCT

  • Size:

    23

  • Amplicon:

  • Sequence:

    tcaaatcaacatgggtgactagaaannnncagtactgggcccttgtggcgctnnatcatagctataaacctattcagcacaatg

  • Size:

    84

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-018Link to the document url. EU reference method protocol