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Detection Image Detection method details

Name:

 QT-EVE-GM-013

Description:

 Quantitative real-time PCR (TaqMan) method for the detection of soybean event DAS68416 (EU-RL GMFF GMOMETHODS database as of July 2, 2015)

Comment:

 Target is the 3' integration border region (IBR) between the insert of soybean event DAS68416 and the soybean host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GM-002

Type:

 event-specific

Target GMO name:

 DAS68416

  • Oligonucleotides:

  • Forward Primer

  • Name:

    DAS-68416-4_3f5

  • Sequence:

    GTACATTAAAAACGTCCGCAATGTGT

  • Size:

    26

  • Reverse Primer

  • Name:

    DAS-68416-4_3r3

  • Sequence:

    GTTTAAGAATTAGTTCTTACAGTTTATTGTTAG

  • Size:

    33

  • Probe

  • Name:

    DAS-68416-4_3p3

  • Sequence:

    TTAAGTTGTCTAAGCGTCAATA

  • Size:

    22

  • Amplicon:

  • Sequence:

    gtacattaaaaacgtccgcaatgtgtnnttaagttgtctaagcgtcaatannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnctaacaataaactgtaagaactaattcttaaac

  • Size:

    130

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-013Link to the document url. EU reference method protocol