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Detection Image Detection method details

Name:

 QT-EVE-GH-001b

Description:

 Quantitative real-time PCR (TaqMan) method for detection of cotton event 3006-210-23 (EU-RL GMFF GMOMETHODS database as of October 01, 2014)

Comment:

 Target is the 5' integration border region (IBR) between the insert of cotton event 3006-210-23 and the cotton host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GH-016, QT-TAX-GH-021

Type:

 event-specific

Target GMO name:

 3006-210-23

  • Oligonucleotides:

  • Forward Primer

  • Name:

    3006-f3

  • Sequence:

    AAATATTAACAATGCATTGAGTATGATG

  • Size:

    28

  • Reverse Primer

  • Name:

    3006-r2

  • Sequence:

    ACTCTTTCTTTTTCTCCATATTGACC

  • Size:

    26

  • Probe

  • Name:

    3006-s2

  • Sequence:

    TACTCATTGCTGATCCATGTAGATTTCCCG

  • Size:

    30

  • Amplicon:

  • Sequence:

    aaatattaacaatgcattgagtatgatnnncgggaaatctacatggatcagcaatgagtannnnggtcaatatggagaaaaagaaagagt

  • Size:

    90

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GH-001bLink to the document url. EU reference method protocol