Help icon

Detection Image Detection method details

Name:

 QL-TAX-ZM-003

Description:

 Qualitative conventional PCR method for detection of the maize invertase gene (ISO 21569)

Comment:

 Target is the endogenous invertase (Ivr1) gene from maize (Zea mays)

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 taxon-specific

Target Species name:

 Zea mays

  • Oligonucleotides:

  • Forward Primer

  • Name:

    IVR1-F

  • Sequence:

    CCGCTGTATCACAAGGGCTGGTACC

  • Size:

    25

  • Reverse Primer

  • Name:

    IVR1-R

  • Sequence:

    GGAGCCCGTGTAGAGCATGACGATC

  • Size:

    25

  • Amplicon:

  • Sequence:

    ccgctgtatcacaagggctggtaccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngatcgtcatgctctacacgggctcc

  • Size:

    225

Documents

Citation Type Local copy
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods ISO 21569:1-69 (2005)Link to the document url. other document
EU-RL GMFF GMOMETHODS database; QL-TAX-ZM-003Link to the document url. EU reference method protocol
Pan L., Zhang S., Yang L., Broll H., Tian F., Zhang D.; Interlaboratory trial validation of an event-specific qualitative polymerase chain reaction-based detection method for genetically modified RT73 rapeseed J AOAC Int 90:1639-1646 (2007)Link to the document url. peer-reviewed article