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Detection Image Detection method details

Name:

 QL-ELE-00-001

Description:

 Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31)

Comment:

 Target is the Cauliflower mosaic virus 35S promoter (CaMV P-35S)

Validation:

 ring trial validation

Standardisation:

 national standard

Related Methods:

Type:

 element-specific

Target DNA element:
P-Cauliflower mosaic virus

  • Oligonucleotides:

  • Forward Primer

  • Name:

    35S-1

  • Sequence:

    GCTCCTACAAATGCCATCA

  • Size:

    19

  • Reverse Primer

  • Name:

    35S-2

  • Sequence:

    GATAGTGGGATTGTGCGTCA

  • Size:

    20

  • Amplicon:

  • Sequence:

    -

  • Size:

    195

Documents

Citation Type Local copy