Detection method details
Name: |
QL-TAX-LU-001 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection flax stearoyl-acyl carrier protein desaturase gene of flax/linseed (EU-RL GMFF GMOMETHODS database as of January 16, 2020) |
Comment: |
Target is the endogenous stearoyl-acyl carrier protein desaturase gene (sad) of Linum usitatissimum |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Linum usitatissimum |
-
Oligonucleotides:
Forward Primer
Name:
SAD-FWSequence:
GCTCAACCCAGTCACCACCTSize:
20
Reverse Primer
Name:
SAD-RVSequence:
TGCGAGGAGATCTGGAGGAGSize:
20
Probe
Name:
SAD-probeSequence:
TGTTGAGGGAGCGTGTTGAAGGGASize:
24
-
Amplicon:
Sequence:
gctcaaccca gtcaccacct tcccttcaac acgctccctc aacannnnct cctccagatc tcctcgcaSize:
68
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QL-TAX-LU-001 | EU reference method protocol |