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Detection Image Detection method details

Name:

 QT-EVE-ZM-023

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event Bt176 (EU-RL GMFF GMOMETHODS database as of October 01, 2014).

Comment:

 Target is the 3' integration border region (IBR) between the insert of maize event Bt176 and the maize host genome.

Validation:

 in-house validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-014

Type:

 event-specific

Target GMO name:

 Bt176

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Bt176 F

  • Sequence:

    GGCCGTGAACGAGCTGTT

  • Size:

    18

  • Reverse Primer

  • Name:

    Bt176 R

  • Sequence:

    GGGAAGAAGCCTACATGTTTTCTAA

  • Size:

    25

  • Probe

  • Name:

    Bt176 Probe

  • Sequence:

    AGCAACCAGATCGGCCGACACC

  • Size:

    22

  • Amplicon:

  • Sequence:

    ggccgtgaacgagctgttnnnnnnnagcaaccagatcggccgacaccnnnnnnnnnnttagaaaacatgtaggcttcttccc

  • Size:

    82

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-023Link to the document url. EU reference method protocol