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Detection Image Detection method details

Name:

 QT-EVE-GM-015

Description:

 Quantitative PCR method for detection of soybean event DAS44406 (EURL GMFF GMOMETHODS database as of May 28, 2015)

Comment:

 Target is the 5' integration border region (IBR) between the insert of soybean event DAS44406 and the soybean host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GM-002

Type:

 event-specific

Target GMO name:

 DAS44406

  • Oligonucleotides:

  • Forward Primer

  • Name:

    DAS-44406-5F

  • Sequence:

    TTATTGTTCTTGTTGTTTCCTCTTTAGG

  • Size:

    28

  • Reverse Primer

  • Name:

    DAS-44406-5R

  • Sequence:

    CCTCAATTGCGAGCTTTCTAATTT

  • Size:

    24

  • Probe

  • Name:

    DAS-44406-6-5p1

  • Sequence:

    ATTCGGACCTCCATGATGACCTTACCGTT

  • Size:

    29

  • Amplicon:

  • Sequence:

    ttattgttcttgttgtttcctctttaggnnnnnnnnnnnnaacggtaaggtcatcatggaggtccgaatnnnnnnaaattagaaagctcgcaattgagg

  • Size:

    99

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-015Link to the document url. EU reference method protocol