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Detection Image Detection method details

Name:

 QT-EVE-GM-011

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean event CV127 (EU-RL GMFF GMOMETHODS database as of January 23, 2014)

Comment:

 Target is the 3' integration border region (IBR) between the insert of soybean event CV127 and the soybean host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GM-002

Type:

 event-specific

Target GMO name:

 CV127

  • Oligonucleotides:

  • Forward Primer

  • Name:

    SE-127-f4

  • Sequence:

    AACAGAAGTTTCCGTTGAGCTTTAAGAC

  • Size:

    28

  • Reverse Primer

  • Name:

    SE-127-r2

  • Sequence:

    CATTCGTAGCTCGGATCGTGTAC

  • Size:

    23

  • Probe

  • Name:

    SE-127-p3

  • Sequence:

    TTTGGGGAAGCTGTCCCATGCCC

  • Size:

    23

  • Amplicon:

  • Sequence:

    aacagaagtttccgttgagctttaagacntttggggaagctgtcccatgcccnnnnnnnnnnnnngtacacgatccgagctacgaatg

  • Size:

    88

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-011Link to the document url. EU reference method protocol