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Detection Image Detection method details

Name:

 QT-EVE-GM-008

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean event DP305423 (EU-RL GMFF GMOMETHODS database as of January 23, 2014)

Comment:

 Target is the 3' integration border region (IBR) between the insert of soybean event DP305423 and the soybean host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GM-002

Type:

 event-specific

Target GMO name:

 DP305423

  • Oligonucleotides:

  • Forward Primer

  • Name:

    DP305-f1

  • Sequence:

    CGTGTTCTCTTTTTGGCTAGC

  • Size:

    21

  • Reverse Primer

  • Name:

    DP305-r5

  • Sequence:

    GTGACCAATGAATACATAACACAAACTA

  • Size:

    28

  • Probe

  • Name:

    DP305-p

  • Sequence:

    TGACACAAATGATTTTCATACAAAAGTCGAGA

  • Size:

    32

  • Amplicon:

  • Sequence:

    cgtgttctctttttggctagcnnnnnnnnnnntctcgacttttgtatgaaaatcatttgtgtcantagtttgtgttatgtattcattggtcac

  • Size:

    93

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-008Link to the document url. EU reference method protocol