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Detection Image Detection method details

Name:

 QL-TAX-OS-006

Description:

 Qualitative conventional PCR method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009).

Comment:

 target is the endogenous sucrose-phosphate synthase (SPS) gene of rice.

BLAST analysis against GenBank (February 2024) shows two mismatches between the sequence of the forward primer and 12 of the 14 listed rice sequences (including the rice reference genome Nipponbare). First analyses performed by WFSR with the PCR-method QT-TAX-OS-003 where the same forward primer is used indicated that an adaptation of the forward primer sequence leads to an increased sensitivity in the detection of several rice varieties.

Validation:

 ring trial validation

Standardisation:

 unknown

Related Methods:

Type:

 taxon-specific

Target Species name:

 Oryza sativa

  • Oligonucleotides:

  • Forward Primer

  • Name:

    SPS-1F

  • Sequence:

    TTGCGCCTGAACGGATAT

  • Size:

    18

  • Reverse Primer

  • Name:

    SPS-2R

  • Sequence:

    GGAGAAGCACTGGACGAGG

  • Size:

    19

  • Amplicon:

  • Sequence:

    ttgcgcctgaacggatatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncctcgtccagtgcttctcc

  • Size:

    277

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-TAX-OS-006Link to the document url. EU reference method protocol
Jiang L., Yang L., Zhang H., Guo J., Mazzara M., Van den Eede G., Zhang D.; International collaborative study of the endogenous reference gene, sucrose phosphate synthase (SPS), used for qualitative and quantitative analysis of genetically modified riceLink to the document url. peer-reviewed article