Detection method details
Name: |
QT-EVE-ZM-019 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize event 3272 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) |
Comment: |
Target is the 5' integration border region (IBR) between the insert of maize event 3272 and the maize host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-ZM-014 |
Type: |
event-specific |
Target GMO name: |
3272 |
-
Oligonucleotides:
Forward Primer
Name:
ES3272-FSequence:
TCATCAGACCAGATTCTCTTTTATGGSize:
26
Reverse Primer
Name:
ES3272-RSequence:
CGTTTCCCGCCTTCAGTTTASize:
20
Probe
Name:
ES3272-PSequence:
ACTGCTGACGCGGCCAAACACTGSize:
23
-
Amplicon:
Sequence:
tcatcagaccagattctcttttatggnnnnnnnnnnnnnnnnnnnnactgctgacgcggccaaacactgnnnnnntaaactgaaggcgggaaacgSize:
95
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-019 | EU reference method protocol |