Detection method details
Name: |
QL-ELE-epsps 1-5'/epsps 3-3' |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the CP4-epsps gene (Scholtens et al. 2013). |
Comment: |
Target is the CP4-epsps gene from bacterium Agrobacterium tumefaciens. |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-CP4epsps-RHIRD
|
-
Oligonucleotides:
Forward Primer
Name:
epsps 1-5'Sequence:
GCCTCGTGTCGGAAAACCCTSize:
20
Reverse Primer
Name:
epsps 3-3'Sequence:
TTCGTATCGGAGAGTTCGATCTTCSize:
24
Probe
Name:
epsps-probeSequence:
TGCCACGATGATCGCCACGAGCTTCCSize:
26
-
Amplicon:
Sequence:
gcctcgtgtcggaaaaccctgtcacggtggacgatgccacgatgatcgccacgagcttcccggagttcatggacctgatggccgggctgggcgcgaagatcgaactctccgatacgaaSize:
118