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Detection method details
Name: |
QL-ELE-00-009 |
Description: |
Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001) |
Comment: |
Target is the nopaline synthase terminator (T-nos) from Agrobacterium tumefaciens |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
T-nos-RHIRD
|
-
Oligonucleotides:
Forward Primer
Name:
HA-nos118-fSequence:
GCATGACGTTATTTATGAGATGGGSize:
24
Reverse Primer
Name:
HA-nos118-rSequence:
GACACCGCGCGCGATAATTTATCCSize:
24
-
Amplicon:
Sequence:
gcatgacgttatttatgagatgggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnggataaattatcgcgcgcggtgtcSize:
118