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Detection Image Detection method details

Name:

 QT-TAX-ZM-014

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

Comment:

 Target is the endogenous alcohol deydrogenase 1 (adh1) gene from Zea mays. This assay is recommended for GMO quantitative analysis.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 taxon-specific

Target Species name:

 Zea mays

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Zm Adh1 primer F

  • Sequence:

    CGTCGTTTCCCATCTCTTCCTCC

  • Size:

    23

  • Reverse Primer

  • Name:

    Zm Adh1 primer R

  • Sequence:

    CCACTCCGAGACCCTCAGTC

  • Size:

    20

  • Probe

  • Name:

    Zm Adh1

  • Sequence:

    AATCAGGGCTCATTTTCTCGCTCCTCA

  • Size:

    27

  • Amplicon:

  • Sequence:

    cgtcgtttcccatctcttcctccnnnnnnnnnnnnnnnnnnnnaatcagggctcattttctcgctcctcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngactgagggtctcggagtgg

  • Size:

    135

Documents

Citation Type Local copy
Jacchia S., Kagkli D.F., Lievens A., Angers-Loustau A., Savini C., Emons H., Mazzara M. (2018) Identification of single target taxon-specific reference assays for the most commonly genetically transformed crops using digital droplet PCR. Food Control 93,191-200Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QT-TAX-ZM-014Link to the document url. EU reference method protocol