Help icon

Detection Image Detection method details

Name:

 QT-EVE-ZM-004

Description:

 Quantitative real-time PCR (TaqMan) method for maize event DAS40278 (EU-RL GMFF GMOMETHODS database as of October 01, 2014)

Comment:

 Target is the 5' integration border region (IBR) between the insert of maize event DAS40278 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-002

Type:

 event-specific

Target GMO name:

 DAS40278

  • Oligonucleotides:

  • Forward Primer

  • Name:

    DAS-40278-9_5'-f1

  • Sequence:

    CACGAACCATTGAGTTACAATC

  • Size:

    22

  • Reverse Primer

  • Name:

    DAS-40278-9_5'-r3

  • Sequence:

    TGGTTCATTGTATTCTGGCTTTG

  • Size:

    23

  • Probe

  • Name:

    DAS-40278-9_5'-S2

  • Sequence:

    CGTAGCTAACCTTCATTGTATTCCG

  • Size:

    25

  • Amplicon:

  • Sequence:

    cacgaaccattgagttacaatcnnnnnnnnnnnnnnnnnnnncggaatacaatgaaggttagctacgnnnnnnnncaaagccagaatacaatgaacca

  • Size:

    98

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-004Link to the document url. EU reference method protocol