Detection method details
Name: |
QT-EVE-BN-006 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf1 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) |
Comment: |
Target is the 5' integration border region (IBR) between the insert of oilseed rape event Rf1 and the oilseed rape host genome |
Validation: |
in-house validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-BN-012 |
Type: |
event-specific |
Target GMO name: |
Rf1 |
-
Oligonucleotides:
Forward Primer
Name:
MDB118Sequence:
CTAAGGGAGGTCAAGATGTAGCSize:
22
Reverse Primer
Name:
KVM170Sequence:
CGGGCCTAACTTTTGGTGTGSize:
20
Probe
Name:
TM022Sequence:
CTCATCATCCTCACCCAGTCAGCATCASize:
27
-
Amplicon:
Sequence:
ctaagggaggtcaagatgtagcnnnnnnnnaacccttttccatcttttccggtgactacannnnnctcatcatcctcacccagtcagcatcancacaccaaaagttaggcccgSize:
113
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-006 | EU reference method protocol |